The Effect of Endogenous Adenosine on Neuronal Activity in Rats: An FDG PET Study.
Identifieur interne : 000179 ( Main/Exploration ); précédent : 000178; suivant : 000180The Effect of Endogenous Adenosine on Neuronal Activity in Rats: An FDG PET Study.
Auteurs : Fiona E. Parkinson [Canada] ; Soumen Paul [Canada] ; Dali Zhang [Canada] ; Shadreck Mzengeza [Canada] ; Ji Hyun Ko [Canada]Source :
- Journal of neuroimaging : official journal of the American Society of Neuroimaging [ 1552-6569 ] ; 2016.
Abstract
2-(18) F-fluorodeoxy-D-glucose (FDG) is a glucose analog that is taken up by cells and phosphorylated. The amount of FDG accumulated by cells is a measure of the rate of glycolysis, which reflects cellular activity. As the levels and actions of the neuromodulator adenosine are dynamically regulated by neuronal activity, this study was designed to test whether endogenous adenosine affects tissue accumulation of FDG as assessed by positron emission tomography (PET) or by postmortem analysis of tissue radioactivity. Rats were given an intraperitoneal injection of the adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropyl-xanthine (DPCPX, 3 mg/kg), the adenosine kinase inhibitor ABT-702 (3 mg/kg), or vehicle 10 minutes prior to an intravenous injection of FDG (15.4 ± 0.7 MBq per rat). Rats were then subjected to a 15 minute static PET scan. Reconstructed images were normalized to FDG PET template for rats and standard uptake values (SUVs) were calculated. To examine the regional effect of active treatment compared to vehicle, statistical parametric mapping analysis was performed. Whole-brain FDG uptake was not affected by drug treatment. Significant regional hypometabolism was detected, particularly in cerebellum, of DPCPX- and ABT-702 treated rats, relative to vehicle-treated rats. Thus, endogenous adenosine can affect FDG accumulation although this effect is modest in quiescent rats.
DOI: 10.1111/jon.12349
PubMed: 27082948
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000206
- to stream PubMed, to step Curation: 000206
- to stream PubMed, to step Checkpoint: 000206
- to stream Ncbi, to step Merge: 001F37
- to stream Ncbi, to step Curation: 001F37
- to stream Ncbi, to step Checkpoint: 001F37
- to stream Main, to step Merge: 000179
- to stream Main, to step Curation: 000179
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Effect of Endogenous Adenosine on Neuronal Activity in Rats: An FDG PET Study.</title><author><name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E" last="Parkinson">Fiona E. Parkinson</name><affiliation wicri:level="4"><nlm:affiliation>Departments of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, MB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Departments of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, MB</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Paul, Soumen" sort="Paul, Soumen" uniqKey="Paul S" first="Soumen" last="Paul">Soumen Paul</name><affiliation wicri:level="4"><nlm:affiliation>Departments of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, MB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Departments of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, MB</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Zhang, Dali" sort="Zhang, Dali" uniqKey="Zhang D" first="Dali" last="Zhang">Dali Zhang</name><affiliation wicri:level="4"><nlm:affiliation>Departments of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Departments of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Mzengeza, Shadreck" sort="Mzengeza, Shadreck" uniqKey="Mzengeza S" first="Shadreck" last="Mzengeza">Shadreck Mzengeza</name><affiliation wicri:level="4"><nlm:affiliation>Departments of Radiology, University of Manitoba, Winnipeg, MB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Departments of Radiology, University of Manitoba, Winnipeg, MB</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Ko, Ji Hyun" sort="Ko, Ji Hyun" uniqKey="Ko J" first="Ji Hyun" last="Ko">Ji Hyun Ko</name><affiliation wicri:level="4"><nlm:affiliation>Departments of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Departments of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27082948</idno><idno type="pmid">27082948</idno><idno type="doi">10.1111/jon.12349</idno><idno type="wicri:Area/PubMed/Corpus">000206</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000206</idno><idno type="wicri:Area/PubMed/Curation">000206</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000206</idno><idno type="wicri:Area/PubMed/Checkpoint">000206</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000206</idno><idno type="wicri:Area/Ncbi/Merge">001F37</idno><idno type="wicri:Area/Ncbi/Curation">001F37</idno><idno type="wicri:Area/Ncbi/Checkpoint">001F37</idno><idno type="wicri:Area/Main/Merge">000179</idno><idno type="wicri:Area/Main/Curation">000179</idno><idno type="wicri:Area/Main/Exploration">000179</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The Effect of Endogenous Adenosine on Neuronal Activity in Rats: An FDG PET Study.</title><author><name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E" last="Parkinson">Fiona E. Parkinson</name><affiliation wicri:level="4"><nlm:affiliation>Departments of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, MB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Departments of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, MB</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Paul, Soumen" sort="Paul, Soumen" uniqKey="Paul S" first="Soumen" last="Paul">Soumen Paul</name><affiliation wicri:level="4"><nlm:affiliation>Departments of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, MB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Departments of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, MB</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Zhang, Dali" sort="Zhang, Dali" uniqKey="Zhang D" first="Dali" last="Zhang">Dali Zhang</name><affiliation wicri:level="4"><nlm:affiliation>Departments of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Departments of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Mzengeza, Shadreck" sort="Mzengeza, Shadreck" uniqKey="Mzengeza S" first="Shadreck" last="Mzengeza">Shadreck Mzengeza</name><affiliation wicri:level="4"><nlm:affiliation>Departments of Radiology, University of Manitoba, Winnipeg, MB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Departments of Radiology, University of Manitoba, Winnipeg, MB</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Ko, Ji Hyun" sort="Ko, Ji Hyun" uniqKey="Ko J" first="Ji Hyun" last="Ko">Ji Hyun Ko</name><affiliation wicri:level="4"><nlm:affiliation>Departments of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Departments of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author></analytic><series><title level="j">Journal of neuroimaging : official journal of the American Society of Neuroimaging</title><idno type="eISSN">1552-6569</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">2-(18) F-fluorodeoxy-D-glucose (FDG) is a glucose analog that is taken up by cells and phosphorylated. The amount of FDG accumulated by cells is a measure of the rate of glycolysis, which reflects cellular activity. As the levels and actions of the neuromodulator adenosine are dynamically regulated by neuronal activity, this study was designed to test whether endogenous adenosine affects tissue accumulation of FDG as assessed by positron emission tomography (PET) or by postmortem analysis of tissue radioactivity. Rats were given an intraperitoneal injection of the adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropyl-xanthine (DPCPX, 3 mg/kg), the adenosine kinase inhibitor ABT-702 (3 mg/kg), or vehicle 10 minutes prior to an intravenous injection of FDG (15.4 ± 0.7 MBq per rat). Rats were then subjected to a 15 minute static PET scan. Reconstructed images were normalized to FDG PET template for rats and standard uptake values (SUVs) were calculated. To examine the regional effect of active treatment compared to vehicle, statistical parametric mapping analysis was performed. Whole-brain FDG uptake was not affected by drug treatment. Significant regional hypometabolism was detected, particularly in cerebellum, of DPCPX- and ABT-702 treated rats, relative to vehicle-treated rats. Thus, endogenous adenosine can affect FDG accumulation although this effect is modest in quiescent rats.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Manitoba</li></region><settlement><li>Winnipeg</li></settlement><orgName><li>Université du Manitoba</li></orgName></list><tree><country name="Canada"><region name="Manitoba"><name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E" last="Parkinson">Fiona E. Parkinson</name></region><name sortKey="Ko, Ji Hyun" sort="Ko, Ji Hyun" uniqKey="Ko J" first="Ji Hyun" last="Ko">Ji Hyun Ko</name><name sortKey="Mzengeza, Shadreck" sort="Mzengeza, Shadreck" uniqKey="Mzengeza S" first="Shadreck" last="Mzengeza">Shadreck Mzengeza</name><name sortKey="Paul, Soumen" sort="Paul, Soumen" uniqKey="Paul S" first="Soumen" last="Paul">Soumen Paul</name><name sortKey="Zhang, Dali" sort="Zhang, Dali" uniqKey="Zhang D" first="Dali" last="Zhang">Dali Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000179 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000179 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Canada
|area= ParkinsonCanadaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:27082948
|texte= The Effect of Endogenous Adenosine on Neuronal Activity in Rats: An FDG PET Study.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27082948" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ParkinsonCanadaV1
| This area was generated with Dilib version V0.6.29. |  |